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结合大分子自组装和分子印迹技术制备了分子印迹聚合物胶束,并通过电沉积将其固载到电极表面得到分子印迹电化学传感器.首先以甲基丙烯酸二甲氨基乙酯(DMA)、丙烯酸羟乙酯(HEA)、丙烯酸二异辛酯(EHA)和苯乙烯(St)合成了共聚物poly(DMA-co-HEA-co-EHA-co-St),在其侧链接枝上双键得到可光交联的双亲共聚物.以水为沉淀剂诱发该双亲共聚物在含有对乙酰氨基苯酚的溶液中自组装,得到印迹有对乙酰氨基苯酚的聚合物胶束,利用动态激光光散射(DLS)和透射电镜(TEM)表征其尺寸和形貌.最后通过电沉积技术诱导印迹胶束在金电极表面组装,经紫外光辐照交联后,洗脱模板分子形成分子印迹膜,制备了对乙酰氨基苯酚分子印迹传感器,通过循环伏安法、差分脉冲溶出伏安法研究此印迹传感器的性能.实验结果表明,该传感器对对乙酰氨基苯酚具有良好的选择性和灵敏度,浓度响应线性范围为1×10-6到4×10-3mol/L,检测限为3.3×10-7mol/L.
Molecularly imprinted polymer micelles were prepared by macromolecule self-assembly and molecular imprinting technology and immobilized on the electrode surface by electrodeposition to obtain molecularly imprinted electrochemical sensor.At first, DMA, The copolymer poly (DMA-co-HEA-co-EHA-co-St) was synthesized by using hydroxyethyl acrylate (HEA), diisooctyl acrylate (EHA) and styrene (St) Bond to obtain a photocrosslinkable amphiphilic copolymer.With water as a precipitating agent, the amphiphilic copolymer was induced to self-assemble in a solution containing acetaminophen to obtain a polymer micelles imprinted with acetaminophen, and a dynamic laser light (DLS) and transmission electron microscopy (TEM) .Finally, the electrodeposited micelles were used to induce the formation of imprinted micelles on the surface of gold electrode. After crosslinking by ultraviolet light, the template molecules were eluted to form molecular imprinted membrane. The acetaminophen molecular imprinted sensor was prepared and its performance was studied by cyclic voltammetry and differential pulse stripping voltammetry.The experimental results show that the sensor has good selectivity and sensitivity to acetaminophen, The range of 1 × 10-6 to 4 × 10-3mol / L, the detection limit was 3.3 × 10-7mol / L.