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本文作者首次报告了从扎伊尔人穿刺的皮肤活组织标本中,成功地获得3条完整的链尾棘唇丝虫(Dipetalonema streptocerca)成虫(2条雌虫,1条雄虫),另获得6条雌虫的断片。给病人一次口服50毫克海群生后,24小时内在皮肤出现的结节处作活组织穿刺(7毫米),然后将其固定在硫酸铵溶液内。切除后7～14天,标本经生理缓冲盐水清洗30分钟,放在1%胶原酶tris—盐酸缓冲液(pH7.4)内,置于室温(25℃)孵育2～3天。然后
The authors report for the first time that three complete adults of Dipetalonema streptocerca (two females and one male) have been successfully obtained from skin biopsies punctured by Zairian people and were also obtained Pieces of 6 females. Once given orally to a patient of 50 mg of sea lions, biopsies (7 mm) were made in the nodules of the skin within 24 hours and then fixed in ammonium sulfate solution. After 7 to 14 days of resection, specimens were washed with physiological saline for 30 minutes and placed in 1% collagenase tris-hydrochloric acid buffer (pH 7.4) and incubated at room temperature (25 ° C) for 2 to 3 days. then