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紫花苜蓿不同部位经一定处理及显色后于５４５ｎｍ处有最大吸收，利用这一特性，以比色法测定了紫花苜蓿不同部位总皂甙含量，回收率９７．１％，ＲＳＤ＝０．０１２（ｎ＝５），符合质控要求。
Different parts of alfalfa had maximum absorption at 545 nm after a certain treatment and color development. Using this property, the total saponin content in different parts of alfalfa was determined by colorimetric method, with a recovery rate of 97.1% and RSD=0.012 ( n = 5), in line with quality control requirements.