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乙型肝炎病毒（ＨＢＶ）Ｐｒｅ－Ｓ蛋白是ＨＢＶ与靶细胞结合的重要蛋白。ＩＦＮ是生物体内具有多种生物功能的细胞因子，ＩＦＮ在乙型肝炎的防治中具有重要意义。通过ＰＣＲ技术，我们在体外扩增了ＩＦＮαＡ基因的全序列和ＨＢＶＰｒｅ－Ｓ基因的全序列。为了便于融合基因的克隆和表达，我们在引物的５’端设计了相应的酶切位点，保护位点，起始密码和终止密码。通过基因克隆技术，我们构建了ＩＦＮαＡ－ＨＢＶＰｒｅ－Ｓ融合基因的克隆并进行了序列测定。大量研究表明，ＨＢＶ和靶细胞的结合可能是由ＨＢＶ包膜上的Ｐｒｅ－Ｓ蛋白表位介导的，因此带有附着位点的Ｐｒｅ－Ｓ蛋白，可能比ＨＢｓＡｇ疫苗保护效果好。另一方面，α－干扰素作为治疗乙型肝炎的重要生物制剂，也是乙肝免疫接种过程中良好的免疫性剂。ＩＦＮαＡ－ＨＢＶＰｒｅ－Ｓ融合基因克隆的成功，为表达同时具有ＩＦＮαＡ生物学活性以及ＨＢＶＰｒｅ－Ｓ蛋白免疫原性的双特性蛋白奠定了基础。
Hepatitis B Virus (HBV) Pre-S protein is an important protein of HBV that binds to target cells. IFN is a biological factor in vivo with a variety of biological cytokines, IFN in the prevention and treatment of hepatitis B is of great significance. Through PCR, we amplified the complete sequence of IFNαA gene and the complete sequence of HBV Pre-S gene in vitro. In order to facilitate the cloning and expression of the fusion gene, we designed the corresponding restriction sites, protection sites, start codon and stop codon at the 5 ’end of the primer. By gene cloning technology, we constructed a clone of IFNαA-HBVPre-S fusion gene and sequenced. Numerous studies have shown that the binding of HBV to target cells may be mediated by Pre-S protein epitopes on the HBV envelope, and therefore Pre-S proteins with attachment sites may be more potent than HBsAg vaccines. On the other hand, α-interferon, as an important biological agent for the treatment of hepatitis B, is also a good immunological agent during hepatitis B immunization. The success of the cloning of the IFNαA-HBV Pre-S fusion gene lays the foundation for the expression of a bi-specific protein that has both the IFNαA biological activity and the immunogenicity of the HBV Pre-S protein.