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收集河南省1 038份HIV携带者外周血样,提取其基因组DNA后,利用巢式PCR技术扩增弓形虫529 bp重复序列基因,凝胶电泳检测目的片段。1 038份HIV携带者全血血样中(男762份,女276份),弓形虫PCR阳性血样66份,弓形虫PCR阳性率为6.4%(66/1 038)。男性和女性的弓形虫PCR阳性率分别为6.3%(48/762)和6.5%(18/276)。已婚508份,未婚、离异或丧偶530份,其弓形虫PCR阳性率分别为4.9%(25/508)和7.7%(41/530)(χ2=3.451,P>0.05)。高中及以上学历、高中以下学历人群的弓形虫PCR阳性率分别为6.9%(34/489)和5.8%(32/549)(χ2=0.545,P>0.05)。20~40岁组人群的弓形虫PCR阳性率最高,为7.6%(31/410)。有性病史、无性病史和病史不详者的弓形虫PCR阳性率分别为8.0%(9/113)、6.5%(50/773)和4.6%(7/152)(χ2=0.355,P>0.05)。
Peripheral blood samples from 1 038 HIV carriers in Henan Province were collected and their genomic DNA was extracted. The 529 bp repeat DNA of Toxoplasma gondii was amplified by nested PCR, and the target fragment was detected by gel electrophoresis. Totally 662 HIV samples were obtained from 1 038 HIV-infected whole blood samples (762 males and 276 females), 66 PCR-positive blood samples from Toxoplasma gondii, and 6.4% (66/1 038) PCR-positive to Toxoplasma gondii. The positive rates of Toxoplasma gondii PCR in male and female were 6.3% (48/762) and 6.5% (18/276) respectively. 508 were married, 530 were unmarried, divorced or widowed. The positive rate of Toxoplasma gondii PCR was 4.9% (25/508) and 7.7% (41/530) respectively (χ2 = 3.451, P> 0.05). The highest positive rates of Toxoplasma gondii PCR were 6.9% (34/489) and 5.8% (32/549), respectively (χ2 = 0.545, P> 0.05). The highest positive rate of Toxoplasma gondii PCR was found in 20-40 age group (7.6%, 31/410). The positive rate of Toxoplasma gondii with the history of venereal disease, history of asexual disease and unknown history were 8.0% (9/113), 6.5% (50/773) and 4.6% (7/152) respectively (χ2 = 0.355, P> 0.05) .