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隐孢子虫病是婴幼儿腹泻的重要病原之一。为了解鲁中南地区隐孢子虫的感染情况,作者于1989～1990年10月进行了调查,结果报告如下。一、材料与方法(一)粪便标本的采集和保存收集泰安、济南、德州、聊城地区以及苍山、邹县等地医院儿科腹泻患儿粪便标本;并对邹县部分农村居民、学龄儿童,以及≤5岁婴幼儿进行普查。采集粪便,置于10％福尔马林1ml 的洁净的空青霉素小瓶内,带回实验室保存待检。(二)粪检用棉棒取少量粪便,在洁净的玻片上涂成薄膜,待干后用甲醇固定,用改良抗酸染色法染色,油镜下检查卵囊,并以每油镜视野查到0～2个卵囊者定
Cryptosporidiosis is one of the important pathogens in infantile diarrhea. In order to understand the infection of Cryptosporidium in Central and Southern Shandong, the author conducted a survey from 1989 to October 1990 and the results are reported as follows. Materials and Methods (1) Collecting and Preservation of Stool Specimen Stool samples of children with pediatric diarrhea in hospitals in Tai’an, Jinan, Dezhou, Liaocheng and Cangshan and Zouxian counties were collected. Some rural residents, school-age children in Zou county, ≤ 5-year-old infant census. Collect faeces, placed in 10ml formalin 1ml of clean empty penicillin vials, back to the laboratory for inspection. (B) fecal examination with a small amount of swab to take a small amount of feces, in a clean slide coated with a thin film, to be dry and fixed with methanol, with improved acid stain staining, oyster examination of oocysts, and each oil field of vision inspection To 0 to 2 oocysts were set