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利用大鼠在体单向肠灌流模型,采用HPLC测定灌流液中刺芒柄花素含量,分别考察刺芒柄花素质量浓度、不同肠段以及P-糖蛋白抑制剂对刺芒柄花素肠吸收的影响,得出丰城鸡血藤中刺芒柄花素在大鼠体内的肠吸收机制。实验结果显示灌流液中刺芒柄花素质量浓度对吸收速率常数(K a)和表观吸收系数(P app)均无显著性影响;刺芒柄花素在小肠段(十二指肠、空肠和回肠)的K a和P app无显著性差异显著,但其K a显著大于在结肠处的值(P<0.05),小肠段和结肠段的P app无显著性差异;P-糖蛋白抑制剂维拉帕米对刺芒柄花素在各肠段的K a和P app均有显著性差异(P<0.05)。表明刺芒柄花素在大鼠肠道内的吸收机制为被动扩散,不存在饱和吸收;其在全肠段的吸收较好,吸收窗主要在小肠,且小肠内无明显的特定吸收部位;刺芒柄花素可能是P-糖蛋白的底物。
The rat mononuclear intestinal perfused rat model was used to determine the content of thymisin in perfused solution by HPLC. The concentration of thymus flower thunb, the different intestinal segments and P-glycoprotein inhibitor Gut absorption, we got the intestinal absorption mechanism of Thorlapunat in Fengcheng Millettia in rats. The experimental results showed that there was no significant difference in absorption rate constant (K a) and apparent absorption coefficient (P app) between the concentration of thymonium and actin in perfusate. In the small intestine (duodenum, Jejunum and ileum). However, the K a was significantly greater than that in the colon (P <0.05), while there was no significant difference in P app between the small intestine and the colon. The P-glycoprotein Inhibitor verapamil had a significant difference in K a and P app (P <0.05) between each step. The results showed that the absorption mechanism of thornonon in rat intestine was passive diffusion and there was no saturation absorption. The absorption was better in the whole bowel segment, the absorption window was mainly in the small intestine, and there was no obvious specific absorption site in the small intestine. Menonnisin may be P-glycoprotein substrate.