论文部分内容阅读
目的全面认识肾移植急性排斥反应早期移植物内基因表达改变,为进一步探讨肾移植急性排斥反应的发病机理、寻找早期诊断指标提供实验依据。方法以F344大鼠为供者,近交系Lewis大鼠为受者,建立大鼠原位肾移植急性排斥反应模型(同种异体移植组),以近交系Lewis大鼠间肾移植为对照(近交系移植组)。观察术后3、7和14 d时的移植肾组织病理学改变,并利用基因芯片技术检测7 d时移植物中基因表达水平,获得其表达谱。结果急性排斥反应早期存在大量基因变化,在两个通道均有有效杂交信号的基因中,上调的有48条,其中γ干扰素诱导GTP酶基因上调最为显著。结论移植物排斥反应是个多途径连续衍变的动态不平衡过程,诸多促进排斥与保护因子并存,很难以单一标志物对肾移植急性排斥反应进行早期诊断,但同时通过多个标志物来综合分析还是有一定可能性的。
Objective To fully understand the changes of gene expression in early stage of acute rejection after renal transplantation, and to provide experimental evidence for further exploring the pathogenesis of acute rejection of renal allograft and searching for early diagnosis indexes. Methods Acute rejection model of orthotopic kidney transplantation (allograft group) was established by using F344 as donor and inbred Lewis rats as recipients. The inbred Lewis rats were used as control Inbred line transplantation group). The pathological changes of renal allografts at 3, 7 and 14 days after operation were observed. The expression of gene in 7-day-old grafts was detected by gene chip technique. Results There were a large number of gene alterations in acute rejection. There were 48 up-regulated genes in both channels, and the upregulation of γ-interferon-induced GTPase gene was the most significant. CONCLUSION: Allograft rejection is a dynamic unbalanced process of continuous evolution. Many of them promote the coexistence of rejection and protection factors. It is difficult to diagnose acute rejection of renal allograft with single marker. However, There is a certain possibility.