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用反相高效液相色谱法测定藏药生等心材中墨沙酮的含量。浸泡和超声提取相结合,ODS柱,甲醇水(28∶72),内含005mol/L磷酸二氢钾(pH4)为流动相,UV290nm检测。回收率为989%。墨沙酮含量为102%。
Determination of Methadone in Heartwood of Tibetan Medicine by Reversed Phase High Performance Liquid Chromatography. ODS column, methanol-water (28:72), containing 0 05mol / L potassium dihydrogen phosphate (pH4) as the mobile phase, UV290nm detection. The recovery rate was 98.9%. Methadone content of 1 02%.