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目的研究外源蔗糖对盐胁迫甘草Glycyrrhiza uralensis幼苗根系生长的缓解效应。方法以甘草幼苗为实验材料,测定不同浓度的外源蔗糖处理下对盐胁迫条件下甘草幼苗生长、有效成分量、可溶性糖量、脯氨酸量及酶活性的影响及测定相关指标,数据采用SPSS 19.0软件分析。结果在盐胁迫条件下,添加一定浓度的外源蔗糖后,甘草幼苗的日相对生长率呈升高趋势;总黄酮量和苯丙氨酸解氨酶(PAL)活性呈极显著升高,外源蔗糖对盐胁迫下甘草幼苗根系生长的缓解作用在很大程度上与PAL活性的增强所引起的黄酮量的增加有关,外源蔗糖的浓度为10 mmol/L时,PAL的活性达到最大,当外源蔗糖的浓度为15 mmol/L时,PAL的活性又成下降趋势;在施加不同浓度的外源蔗糖后甘草酸量比在盐胁迫条件下高,达到未受盐胁迫时的水平,外源蔗糖的浓度梯度对甘草酸积累的影响不明显。脯氨酸和可溶性糖量的变化呈现出相关性,两者皆在施加15 mmol/L的外源蔗糖时达到对照水平,当外源蔗糖的浓度在此基础上增加时,甘草幼苗体内的可溶性糖和脯氨酸的量又呈现上升趋势。超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活性与未经盐胁迫处理的幼苗相比,盐胁迫下幼苗中的SOD及CAT活性均上调,一定浓度的外源蔗糖可降低盐胁迫幼苗中的SOD及CAT活性。结论外源蔗糖可提高甘草幼苗体内抗氧化酶的活性,从而维持了幼苗体内较低的活性氧水平,降低了细胞受过氧化伤害的程度,提高了甘草幼苗对盐胁迫的耐受性。
Aim To study the alleviating effect of exogenous sucrose on root growth of Glycyrrhiza uralensis seedlings under salt stress. Methods Glycyrrhiza uralensis seedlings were used as experimental materials to determine the effects of different concentrations of exogenous sucrose on the growth, content of active ingredients, soluble sugar content, proline content and enzyme activity of Glycyrrhiza uralensis seedlings under salt stress, SPSS 19.0 Software Analysis. Results The relative growth rate of Glycyrrhiza uralensis seedlings showed an increasing trend under the salt stress condition after addition of exogenous sucrose at a certain concentration. The total flavonoid content and PAL activity increased extremely significantly. The allelopathic effects of sucrose on root growth of Glycyrrhiza uralensis seedlings under salt stress were related to the increase of flavonoid content caused by the increase of PAL activity to a large extent. The activity of PAL reached the maximum when the concentration of exogenous sucrose was 10 mmol / L, When the concentration of exogenous sucrose was 15 mmol / L, the activity of PAL decreased again. After the different concentrations of exogenous sucrose were applied, the content of glycyrrhizic acid was higher under salt stress than that under salt stress, The effect of exogenous sucrose concentration gradient on the accumulation of glycyrrhizin is not obvious. The correlation between proline and soluble sugar content showed a correlation, both of which reached the control level when 15 mmol / L exogenous sucrose was applied. When the concentration of exogenous sucrose was increased on the basis of this, the solubility of licorice seedlings in vivo The amount of sugar and proline is on the rise again. The activities of superoxide dismutase (SOD) and catalase (CAT) were higher than those of the seedlings without salt stress. Under the salt stress, the activity of SOD and CAT in the seedlings were increased, Activities of SOD and CAT in Salt Stress Seedlings. Conclusion Exogenous sucrose can increase the activity of antioxidant enzymes in licorice seedlings and thus maintain the low level of reactive oxygen species (ROS) in the seedlings, reduce the degree of oxidative injury and increase the tolerance of licorice seedlings to salt stress.