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目的探讨CD34+细胞在变应性鼻炎患者外周血和鼻黏膜中的表达及髓外CD34+细胞可能参与变应性鼻炎鼻黏膜中的炎性细胞特别是嗜酸粒细胞(eosinophils,EOS)局部生成的可能性。方法取变应性鼻炎发作期和非发作期患者各12例,两组患者分别取外周血,用流式细胞仪做CD34+细胞记数;分别取鼻黏膜,用免疫组化方法标记CD34+细胞,在尽可能排除血管内皮细胞和纤维细胞CD34+阳性表达后,计数CD34+细胞。结果变应性鼻炎患者发作期外周血中CD34+细胞表达明显高于非发作期患者外周血中CD34细胞的表达(P<0.05),鼻黏膜中CD34+细胞主要表达于黏膜下层EOS聚集区,非发作期患者鼻黏膜中CD34+细胞表达明显高于发作期的鼻黏膜(P<0.01),并与活化的EOS数呈负相关。结论变应性鼻炎外周血、鼻黏膜中CD34+细胞的表达,提示了髓外CD34+细胞可能参与变应性鼻炎鼻黏膜中的炎性细胞特别是EOS的局部生成。
Objective To investigate the expression of CD34 + cells in peripheral blood and nasal mucosa of patients with allergic rhinitis and extramedullary CD34 + cells may be involved in the local production of inflammatory cells, especially eosinophils (EOS) in the nasal mucosa of allergic rhinitis possibility. Methods Twelve patients with allergic rhinitis were included in each episode and non-episode. CD34 + cells were counted by flow cytometry in peripheral blood and nasal mucosa respectively. CD34 + cells were labeled by immunohistochemistry, CD34 + cells were counted after eliminating the CD34 + positive expression of vascular endothelial cells and fibroblasts as much as possible. Results The expression of CD34 + cells in peripheral blood in patients with allergic rhinitis was significantly higher than that in non-attack patients (P <0.05). CD34 + cells in nasal mucosa were mainly expressed in the submucosal EOS aggregates, The expression of CD34 + in nasal mucosa of patients with nasal mucosa was significantly higher than that of nasal mucosa (P <0.01), and negatively correlated with the number of activated EOS. Conclusions The expression of CD34 + cells in peripheral blood and nasal mucosa of allergic rhinitis suggests that extramedullary CD34 + cells may be involved in the local production of inflammatory cells, especially EOS, in the nasal mucosa of allergic rhinitis.