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目的研究邻苯二甲酸二乙基己酯(DEHP)及镉(Cd)对大鼠睾丸巨噬细胞(TM)分泌细胞因子IL-1β的影响。方法利用贴壁法提取大鼠睾丸巨噬细胞。以0.1%二甲基亚砜(DMSO)为对照组,用50μg/ml脂多糖(LPS)作为激活剂,以1μmol/L、10μmol/L、100μmol/L的DEHP或Cd Cl2单独及各自半量联合处理,作用24 h后用ELISA法检测上清液中细胞因子IL-1β的含量;用CCK8法测定各浓度DEHP或Cd Cl2单独处理对细胞活性的影响。结果 LPS可明显促进巨噬细胞分泌细胞因子IL-1β;0.5μmol/L DEHP+0.5μmol/L Cd和5μmol/L DEHP+5μmol/L Cd联合染毒可显著抑制细胞分泌功能,但IL-1β分泌量仍明显高于对照组,并介于两单独作用组之间;50μmol/L DEHP+50μmol/L Cd的联合处理可非常显著抑制细胞分泌功能,与对照组相比差异无统计学意义。随着剂量增加,DEHP或Cd单独作用都能抑制细胞分泌功能和细胞活性,但对两者抑制程度存在较大差异。结论 DEHP和Cd联合处理抑制大鼠TM分泌IL-1β表现出一定交互作用,对细胞活性的抑制可部分解释DEHP和Cd对细胞分泌功能的抑制效应。
Objective To study the effect of DEHP and Cd on IL-1β secreted by rat testis macrophages (TMs). Methods The rat testis macrophages were isolated by adherence method. The cells were treated with 50μg / ml lipopolysaccharide (LPS) as activator and 0.1μmol / L, 10μmol / L and 100μmol / L DEHP or CdCl After 24 h treatment, the content of IL-1β in supernatant was detected by ELISA. The effect of DEHP or CdCl 2 alone on cell viability was determined by CCK8 assay. Results LPS could obviously promote the secretion of cytokine IL-1β by macrophages. The combination of 0.5μmol / L DEHP + 0.5μmol / L Cd and 5μmol / L DEHP + 5μmol / L Cd could significantly inhibit the secretion of IL-1β Secretion was still significantly higher than the control group, and between two separate groups; 50μmol / L DEHP + 50μmol / L Cd combination treatment can significantly inhibit cell secretion, compared with the control group, no significant difference. With the increase of dose, DEHP or Cd alone can inhibit the cell secretory function and cell activity, but there is a big difference between the two. Conclusions The combination of DEHP and Cd can inhibit the secretion of IL-1β in rat TM to some extent. The inhibition of cell viability can partly explain the inhibitory effect of DEHP and Cd on the secretion of cells.