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目的:检测尾穗苋(Amaranthus caudatus)甲醇提取物对扑热息痛致大鼠肝损伤的保护作用。方法:5组大鼠分别给予羧甲基纤维素钠、扑热息痛、尾穗苋甲醇提取物(200或400mg/kg)并扑热息痛、水飞蓟素并扑热息痛。给药14d后采集大鼠血样进行血液生化检查,而后处死大鼠,收集各组大鼠肝脏组织样本供组织病理学检查。检测的指标包括肝功能标志物如丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天门冬氨酸氨基转移酶(aspartate aminotransferase,AST)、血清白蛋白(albumin,ALB)、总蛋白(total protein,TP)、总胆红素(total bilirubin,TB)和直接胆红素(direct bilirubin,DB)及氧化损伤标志物如丙二醛(malondialdehyde,MDA)、还原型谷胱甘肽(reduced glutathione,GSH)、总硫醇(total thiols,TT)和过氧化氢酶(catalase,CAT)。结果:与未治疗组相比,浓度为200和400mg/kg的尾穗苋甲醇提取物均能显著改善由扑热息痛引起的大鼠肝损伤,改善扑热息痛导致的ALT、AST、TB和DB水平升高及血清ALB水平降低(P<0.01)。与未治疗组相比,浓度为200和400mg/kg的尾穗苋甲醇提取物能够显著降低扑热息痛导致的MDA及TP水平增高(P<0.01),并显著升高GSH、CAT及TT水平(P<0.01)。大鼠肝脏组织病理学检查的结果也证实了尾穗苋甲醇提取物对大鼠肝脏的保护作用。结论:本研究证实了尾穗苋的甲醇提取物对于扑热息痛引起的大鼠肝损伤具有显著的保护作用,证实了其在民族医学中治疗肝病的药用价值。
Objective: To detect the protective effect of methanol extract from Amaranthus caudatus on liver injury induced by paracetamol in rats. METHODS: Five groups of rats were given carboxymethylcellulose sodium, acetaminophen, and methanol extract (200 or 400 mg/kg), and paracetamol, silymarin and paracetamol. After 14 days of administration, blood samples were collected for blood biochemical examination, and then the rats were sacrificed. The liver tissue samples of each group were collected for histopathological examination. The tested parameters include liver function markers such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (ALB), and total protein (total protein) TP), total bilirubin (TB) and direct bilirubin (DB) and markers of oxidative damage such as malondialdehyde (MDA), reduced glutathione, GSH), total thiols (TT) and catalase (CAT). RESULTS: Compared to the untreated group, methanol extracts of P. fulgrum at concentrations of 200 and 400 mg/kg both significantly improved liver injury induced by paracetamol in rats and improved levels of ALT, AST, TB, and DB due to paracetamol. And serum ALB levels decreased (P <0.01). Compared with the untreated group, the methanol extracts of P. auratus at concentrations of 200 and 400 mg/kg significantly reduced the levels of MDA and TP induced by paracetamol (P<0.01), and significantly increased the levels of GSH, CAT, and TT (P <0.01). The results of histopathological examination of liver in rats also confirmed the protective effect of methanol extract of T. urophylla on rat liver. Conclusion: This study confirms that the methanol extract of T. urophylla has a significant protective effect on paracetamol-induced liver injury in rats, confirming its medicinal value in the treatment of liver disease in ethnic medicine.