论文部分内容阅读
目的探讨VEGFR-3和Prox1在大肠癌组织、正常黏膜组织和淋巴结中的表达及其相互关系。方法采用实时定量RT-PCR检测63份直肠癌远端肠壁组织,156枚系膜淋巴结,配对肿瘤组织和邻近正常黏膜组织各13份的VEGFR-3,Prox1的表达。结果VEGFR-3和Prox1的mRNA含量中位数在肿瘤远端肠壁组织分别为22.08和544.97,两者有明显相关性(r=0.716,P=0.000 1)。在淋巴结分别为33.81和439.16,两者有明显相关性(r=0.618,P=0.000 1)。在肿瘤组织分别为0.65和5877,两者没有相关性(r=-0.280,P=0.353)。结论VEGFR-3与Prox1在正常肠壁组织和系膜淋巴结的新生淋巴管形成中可能有协同作用,但在肿瘤组织不存在协同关系。
Objective To investigate the expression of VEGFR-3 and Prox1 in colorectal carcinoma, normal mucosa and lymph nodes and their correlation. Methods RT-PCR was used to detect the expression of VEGFR-3 and Prox1 in 63 specimens of distal rectal wall, 156 mesangial lymph nodes, 13 matched tissues and 13 adjacent normal mucosa respectively. Results The median mRNA levels of VEGFR-3 and Prox1 were 22.08 and 544.97 respectively in the distal gut tumor tissues (r = 0.716, P = 0.000 1). There were significant correlations between the two in the lymph nodes (r = 0.618, P = 0.0001), 33.81 and 439.16, respectively. The tumor tissues were 0.65 and 5877, respectively, with no correlation (r = -0.280, P = 0.353). Conclusions VEGFR-3 and Prox1 may play a synergistic role in the formation of lymphangiogenesis in normal and mesangial lymph nodes, but there is no synergistic effect in tumor tissues.