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目的胫骨骨髓腔注射法建立前列腺癌PC3骨转移动物模型,采用~(18)F-NaF正电子发射计算机断层显像(PET-CT)核医学影像与X射线平片、显微CT影像等方式监测并比较肿瘤在骨组织的局部生长及其对骨组织的破坏情况。方法肿瘤10只6周龄Balb/c雄性裸鼠麻醉后,从左侧胫骨近端关节面将PC3细胞悬液2μL(2×105细胞)注入胫骨骨髓腔,建立骨转移模型;对照组6只裸鼠从相同位置注入等体积生理盐水。持续记录观察裸鼠的活动及体质量。术前、术后检测血清碱性磷酸酶(ALP)。21d后,裸鼠进行X射线、显微CT及~(18)F-NaF PET-CT检查,观察骨破坏情况。检查完毕后处死裸鼠,取骨组织石蜡包埋切片后经苏木素伊红(HE)染色,在显微镜下观察组织学情况。结果截止实验结束时,10只裸鼠骨转移模型全部成功。肿瘤细胞接种后21d,单纯X射线检查或显微CT无法确诊肿瘤组是否存在骨皮质破坏,但同期~(18)F-NaF PET-CT检查可见肿瘤组胫骨髓腔内出现明显放射性信号,感兴趣区域(ROI)平均标准摄取值最大值SUVmax值为2.10±0.13,高于对照组(0.62±0.14),两者相比差异有统计学意义(P<0.05),提示有肿瘤造成的骨质代谢异常。术后3周肿瘤组血清ALP为(207.87±44.18)U/L,远高于对照组〔(82.75±17.27)U/L〕(P<0.05)。组织病理学检测结果证实肿瘤组胫骨髓腔内出现异常骨质增生。结论利用18F-NaF PET-CT影像监测成功建立了前列腺癌PC-3的早期骨转移动物模型,模拟了前列腺癌对骨组织的成骨侵袭和破坏行为,为进一步研究前列腺癌的早期骨转移机制奠定了基础。
OBJECTIVE: To establish PC3 bone metastasis animal model of prostate cancer by intramedullary injection of tibia and adopt PET-CT nuclear magnetic resonance imaging and X-ray plain film and micro-CT images The local growth of the tumor in the bone tissue and its damage to the bone tissue were monitored and compared. Methods Twenty male Balb / c nude mice, aged 6 weeks, were anesthetized and injected with 2 μL of PC3 cell suspension (2 × 105 cells) into the medullary cavity of the tibia from the proximal articular surface of the left tibia to establish a bone metastasis model. Six rabbits in the control group Nude mice injected an equal volume of saline from the same location. Continue to observe the activity of nude mice and body weight. Preoperative and postoperative serum alkaline phosphatase (ALP). After 21 days, the nude mice were examined by X-ray, micro-CT and ~ (18) F-NaF PET-CT to observe the bone destruction. The nude mice were sacrificed after the examination. The bone tissues were paraffin-embedded and stained with hematoxylin and eosin (HE). The histological changes were observed under a microscope. Results At the end of the experiment, all ten bone metastases in nude mice were successful. Twenty-one days after tumor cell inoculation, X-ray examination or micro-CT could not confirm the existence of cortical destruction in the tumor group. However, significant radioactive signals appeared in the tibial medullary cavity of the tumor group during the same period of ~ (18) F-NaF PET-CT The mean normal SUVmax value of ROI was 2.10 ± 0.13, which was higher than that of the control group (0.62 ± 0.14), the difference was statistically significant (P <0.05), suggesting that the bone mass Metabolic abnormalities. The serum ALP in the tumor group at 3 weeks after operation was (207.87 ± 44.18) U / L, much higher than that in the control group (82.75 ± 17.27 U / L) (P <0.05). Histopathological examination confirmed abnormal bone hyperplasia in the tibial medullary cavity of the tumor group. Conclusion The 18F-NaF PET-CT image monitoring successfully established an early animal model of prostate cancer PC-3 bone metastasis, which simulates the invasion and destruction of bone tissue by prostate cancer. In order to further study the mechanism of early bone metastasis of prostate cancer Foundation.