醋酸棉酚对hCG促进大鼠分散黄体细胞产生孕酮的影响

• 作 者: 郑月慧 吴忠华 方廉
• 机 构: 江西医学院生理教研室,江西医学院生理教研室,江西医学院生理教研室 南昌 330006,南昌 330006,南昌 330006
• 出 处: 药学学报
• 发表日期: 1991年11期
• 关键词: 黄体细胞 hCG 大鼠 棉酚 环磷酸腺苷 作用机制 抑制作用 胚泡着床 细胞存活率 作用时间

本文在体外研究了醋酸棉酚对分散大鼠黄体细胞产生孕酮的影响及其作用机制。当棉酚浓度在20μg/ml、作用时间1h,hCG刺激黄体细胞孕酮的生成量显著下降,但对基础孕酮的产生无明显影响;上述剂量的棉酚在作用4h后时细胞存活率无明显影响。经棉酚作用过的黄体细胞,经2h再孵育,不能恢复其对hCG的反应性。同时棉酚显著降低黄体细胞cAMP产生。结果提示,棉酚可能通过降低cAMP产生而抑制hCG的生孕酮作用。

In this paper, in vitro study of gossypol acetate on rat progesterone production of luteal cells and its mechanism of action. When the concentration of gossypol was 20μg / ml and the action time was 1h, the production of progesterone of luteal cells was significantly decreased by hCG, but there was no significant effect on the production of progesterone. The survival rate of gossypol at 4 hours after treatment Clearly affected. The ghrelin-treated luteal cells, after 2h and then incubated, can not restore its response to hCG. Gossypol also significantly reduced cAMP production in luteal cells. The results suggest that gossypol may inhibit the progesterone production of hCG by decreasing cAMP production.