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银杉(Cathaya argyrophylla)是稀有珍贵植物,有“活化石”之称。目前仅在我国广西、四川、贵州、湖南等地有少量分布。银杉无性繁殖较难,种子繁殖时成苗率也极低。我们用广西花坪所产的种子作材料,初步试验了它的胚培养,愈伤组织的诱导和芽的分化。种子先用95%的酒精浸泡,沉底的种子再用千分之一的昇汞消毒十分钟,然后用无菌水冲洗多次。剥去种皮,接种在改良的 Monnier 培养基上(此培养基原用于银杏的幼胚培养,我们采用它的大量元素成分,微量元素,有机成分和铁二倍于 MS 培养基,蔗糖
Cathaya argyrophylla is a rare and precious plant known as “living fossil.” Currently only in our country Guangxi, Sichuan, Guizhou, Hunan and other places have a small amount of distribution. Silver fir clonal propagation more difficult, seedling seedling rate is also very low. We use the seeds produced in Huaping, Guangxi as a material to preliminary test its embryo culture, callus induction and bud differentiation. The seeds are first soaked in 95% alcohol, the bottom of the seeds is disinfected for ten minutes with one-thousandth of a liter of mercuric chloride, and rinsed several times with sterile water. The seed coat is stripped and inoculated on a modified Monnier medium (which was originally used for immature embryo culture of Ginkgo biloba, we used its large amount of elemental elements, trace elements, organic ingredients and iron twice as much as MS medium, sucrose