论文部分内容阅读
目的采用响应面法对南极磷虾源磷脂的人参皂苷磷脂复合物制备工艺进行优化,并初步探讨其理化性质。方法以人参皂苷复合物的复合率为评估标准,采用单因素及响应面Box-Behnken设计考察各因素对复合物复合率的影响,优化制备工艺。利用扫描电镜观察磷脂复合物的固体粒子表面形态。结果最终影响磷脂复合物复合率的因素有3个:反应时间,人参皂苷浓度,投料比(人参皂苷:磷脂),最佳制备工艺为:以四氢呋喃作为反应溶剂,温度为40℃,反应时间为1.5h,投料比为1∶1.6,人参皂苷浓度为35mg·mL-1,在此条件下复合率可达98.08%.经3次验证实验平均复合率可达97.45%,实验值与预测值之间的相对误差为0.64%。结论响应面法对复合物制备工艺条件进行优化后,提高了复合物制备的复合率。
Objective To optimize the preparation process of ginsenoside phospholipid complex of Antarctic krill derived phospholipids by response surface methodology and to explore its physicochemical properties. Methods The composite rate of ginsenoside complex was taken as the evaluation standard. The single factor and response surface Box-Behnken design was used to investigate the influence of various factors on compound recombination rate and optimize the preparation process. Scanning electron microscopy was used to observe the surface morphology of the solid particles of the phospholipid complex. Results The final results showed that there were three factors affecting the recombination rate of phospholipid complex: reaction time, concentration of ginsenosides and feeding ratio (ginsenoside: phospholipid). The best preparation process was as follows: tetrahydrofuran as reaction solvent, temperature 40 ℃, reaction time 1.5h, the feed ratio was 1: 1.6, the concentration of ginsenoside was 35 mg · mL-1, under this condition the recombination rate could reach 98.08% .The average recombination rate could reach 97.45% after 3 times of validation experiment, the experimental value and the predicted value The relative error between 0.64%. Conclusion Response surface method to optimize the process conditions for the preparation of the composite increases the compounding rate of the preparation.