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目的：通过研究细胞因子和泌乳素（ＰＲＬ）对球后成纤维细胞（ＲＦｓ）表面细胞间粘附分子－１（ＩＣＡＭ－１）表达的影响，进一步了解甲状腺相关眼病（ＴＡＯ）的发病机理，以探索新的防治途径。方法：将体外培养的正常人ＲＦｓ与ＩＦＮ－γ、ＩＬ－４、ＩＦＮ－α和ＰＲＬ孵育７２ｈ，利用直接免疫荧光技术和流式细胞仪检测其表面ＩＣＡＭ－１表达的情况。结果：正常人ＲＦｓ中只有极少数细胞自发并较弱地表达ＩＣＡＭ－１，基础状态下的阳性百分数为（３．４９±１．９９），平均荧光强度为（４４．８４±２０．５９）；而ＩＦＮ－γ、ＩＬ－４、ＩＦＮ－α能以剂量依赖方式显著增强ＩＣＡＭ－１的表达（Ｐ＜０．０５）。ＰＲＬ不仅可以直接刺激ＲＦｓ表面ＩＣＡＭ－１的表达（Ｐ＜０．０５），其剂量相关曲线呈双相型，还能拮抗或协同ＩＦＮ－γ（１００ｕ／ｍｌ）、ＩＦＮ－α（１０００ｕ／ｍｌ）或ＩＬ－４（２．５ｎｇ／ｍｌ）的诱导作用。结论：ＩＦＮ－γ、ＩＬ－４、ＩＦ－α和ＰＲＬ对正常人ＲＦｓ表面ＩＣＡＭ－１的表达有明显的调节作用，它们可能是启动或加重ＴＡＯ自身免疫反应的重要因素。
Objective: To investigate the effects of cytokines and prolactin (PRL) on the expression of intercellular adhesion molecule-1 (ICAM-1) on the surface of retrobulbar fibroblasts (RFs), to further understand the pathogenesis of thyroid-associated ophthalmopathy (TAO) To explore new ways of prevention and control. Methods: The normal human RFs cultured in vitro were incubated with IFN-γ, IL-4, IFN-α and PRL for 72 hours. The expression of ICAM-1 on the surface was detected by direct immunofluorescence and flow cytometry. Results: Only a very few cells in normal human RFs expressed ICAM-1 spontaneously and weakly, the percentage of positive cells was (3.49 ± 1.99) and the average fluorescence intensity was (44.84 ± 20.59) ; While IFN-γ, IL-4 and IFN-α significantly increased ICAM-1 expression in a dose-dependent manner (P <0.05). PRL not only directly stimulated the expression of ICAM-1 on the surface of RFs (P <0.05), but also showed a dose-dependent curve of biphasic type, which could antagonize or cooperate with IFN-γ (100u / ml) ) Or IL-4 (2.5 ng / ml). CONCLUSION: IFN-γ, IL-4, IF-α and PRL have obvious effects on the expression of ICAM-1 on the surface of normal human RFs. They may be important factors in initiating or aggravating TAO autoimmune reaction.