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目的:建立奥沙利铂纳米结构脂质载体(oxaliplatin-loaded nanostuctured lipid carriers,OP-NLC)包封率和含量的测定方法。方法:分别采用葡聚糖凝胶色谱法和超滤法分离OP-NLC中游离药物,高效液相色谱法测定包封率及药物含量。结果:超滤法测得药物包封率略高于葡聚糖凝胶色谱法,平均药物回收率和平均加样回收率分别为(99.3±0.6)%和(99.1±2.2)%。OP-NLC平均包封率为(74.2±1.8)%,药物含量为(0.76±0.03)mg·mL-1。结论:超滤法操作简便、准确,可以用于OP-NLC含量和包封率的测定。
OBJECTIVE: To establish a method for the determination of encapsulation efficiency and content of oxaliplatin-loaded nanostuctured lipid carriers (OP-NLC). Methods: The free drugs in OP-NLC were separated by dextran gel chromatography and ultrafiltration respectively. The entrapment efficiency and drug content were determined by HPLC. Results: The encapsulation efficiency of the drug was slightly higher than that of dextran gel chromatography. The average recovery and the average recovery were (99.3 ± 0.6)% and (99.1 ± 2.2)%, respectively. The average entrapment efficiency of OP-NLC was (74.2 ± 1.8)% and the drug content was (0.76 ± 0.03) mg · mL-1. Conclusion: Ultrafiltration method is simple and accurate and can be used for the determination of OP-NLC content and entrapment efficiency.