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以遗传背景相近、对叶瘟抗性相同但对穗瘟抗性不同的两个水稻株系为材料,利用抑制消减杂交(SSH) 技术构建穗瘟抗／感消减cDNA文库,经差异筛选及序列分析,共获得90个独立的差异表达cDNA克隆,根据与它们同源的基因功能推测,这些克隆可能参与了对病原菌的防卫反应、信号传导和转录等一些重要的生物学过程。利用RT-PCR分析了26个所筛选到的cDNA克隆在抗／感植株接种后的表达,17个基因的表达差异得到验证。对这些差异表达基因在抗感株系接种后不同时间点的表达谱也进行了RT-PCR的分析。文章首次报道了有关水稻对穗瘟抗性在mRNA水平进行研究,为深入研究水稻对穗瘟抗性的遗传机理打下了基础。
Two rice lines with the same resistance to leaf blast but different resistance to panicle blast were used as materials to construct the cDNA library of panicle blast resistant / susceptible subtractive hybridization (SSH) According to their homologous gene function, these clones may be involved in some important biological processes such as defense response to pathogens, signal transduction and transcription. The expression of 26 screened cDNA clones was detected by RT-PCR after inoculation of the resistant / susceptible plants, and the differences of 17 genes were verified. The expression profiles of these differentially expressed genes at different time points after inoculation of resistant lines were also analyzed by RT-PCR. The article reports for the first time that rice resistance to panapoptosis at the mRNA level, which laid the foundation for the further study of the genetic mechanism of panicle blast resistance in rice.