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通过对快慢生型大豆根瘤菌在补充营养液的草炭、蛭石中直接发酵培养58d后,每克蛭石快生菌数可维持到1.8×108,慢生菌数可维持到1.3×108。每克草炭快生菌数为1.7×108,慢生菌数为1.2×108,两种吸附剂保存根瘤菌数都很稳定,但蛭石要优于草炭。而不同接种量的慢生型大豆根瘤菌在草炭、蛭石中发酵40d后,菌数即可达到108/g,表明制备根瘤菌剂时,没必要接入更多的根瘤菌数,菌数的增殖与最初的接种量关系不大。对大豆根瘤菌剂经3年检测结果,有效活菌数都能维持在1.3～1.85×108/g,杂菌数为10%,重金属含量Cd为0.0252mg/kg、Pb为2.99mg/kg、Cr为69.6mg/kg、As为<0.007mg/kg,各项指标均符合国家农业行业制定的有关微生物肥料标准。
After rapid fermentation of soybean rhizobium in peat and vermiculite supplemented with nutrient solution for 58 days, the number of fast-growing bacteria per gram of vermiculite could be maintained to 1.8 × 108, and the number of slow-growing bacteria could be maintained to 1.3 × 108. Per gram of charcoal number of fast-growing bacteria 1.7 × 108, slow-growing bacteria number of 1.2 × 108, two kinds of adsorbents to keep the number of rhizobia are very stable, but vermiculite is better than peat. However, after inoculated with peat and vermiculite for 40 days, the number of bacteria could reach 108 / g, indicating that there is no need to increase the number of rhizobium when preparing rhizobia. The proliferation and the initial inoculum amount of little. After 3 years test, the effective viable count of soybean rhizobia could be maintained at 1.3-1.85 × 108 / g, the number of bacteria was 10%, the content of heavy metal Cd was 0.0252mg / kg, Pb was 2.99mg / kg, Cr 69.6mg / kg, As <0.007mg / kg, the indicators are in line with the national agricultural industry to develop the relevant microbial fertilizer standards.