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采用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)测定不同厂家中/长链脂肪乳注射液中溶血磷脂酰胆碱(LPC)的含量。采用Lichrospher Diol色谱柱(250mm×4.6mm,5μm),以正己烷-异丙醇-水(29.5:59:11.5,V/V/V)为流动相A,正己烷-异丙醇(43:57,V/V)为流动相B,进行梯度洗脱,流速1mL/min,漂移管温度85℃,气体流速2.7L/min。结果溶血磷脂酰胆碱在0.299—2.99mg/mL浓度范围内线性关系良好(r=0.9999),平均回收率为99.62%,RSD为0.89%。不同厂家产品中溶血磷脂酰胆碱的含量为0.92—1.34mg/mL。方法准确、方便,可以准确测定中/长链脂肪乳注射液中溶血磷脂酰胆碱的含量。
The content of lysophosphatidylcholine (LPC) in medium / long-chain fat emulsion injection from different manufacturers was determined by HPLC-ELSD (High Performance Liquid Chromatography-Evaporative Light Scattering Detector). Using hexane-isopropanol-water (29.5: 59: 11.5, V / V / V) as mobile phase A and n-hexane- 57, V / V) as mobile phase B, gradient elution, flow rate 1mL / min, drift tube temperature 85 ℃, gas flow rate 2.7L / min. Results The linear range of lysophosphatidylcholine was 0.299-2.99mg / mL (r = 0.9999). The average recovery was 99.62% and the RSD was 0.89%. The content of lysophosphatidylcholine in different manufacturers was 0.92-1.34mg / mL. The method is accurate and convenient and can accurately determine the content of lysophosphatidylcholine in medium / long-chain fat emulsion injection.