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已有研究认为,胰岛素能促进体外培养的脊髓运动神经元的生长.本研究探讨胰岛素对移植在缺损运动神经元的脊髓内胚胎脊髓腹侧组织块的存活与生长是否也有促进作用.取16只大鼠分为胰岛素组和对照组.两组大鼠在出生后24h内行左侧坐骨神经钳压术,造成脊髓左侧腰段运动神经元缺损.经5～12周后,接受脊髓移植.移植物为13～14d胚龄的大鼠脊髓腹侧组织块,体积为0.3mm~3.移植时,将受体鼠右侧带神经的(足母)长伸肌移植到左侧腰段脊柱与椎旁肌之间,将神经断端与移植物一起置入受体鼠左侧腰段脊髓背外侧部.实
It has been studied that insulin can promote the growth of cultured spinal cord motor neurons in vitro.This study was to investigate whether insulin could also promote the survival and growth of the spinal cord ventral tissue block of spinal cord transplantation in defect motor neurons.Fiveteen The rats were divided into the insulin group and the control group.The left sciatic nerve clamp was performed in the two groups at 24h after birth, resulting in the defect of the left lumbar motor neuron in the spinal cord.After 5 to 12 weeks, the spinal cord was transplanted For 13 ~ 14d embryonic age rat spinal cord ventral tissue mass, the volume of 0.3mm ~ 3. Transplantation, the right side of the recipient rats with nerve (foot) long extensor muscle graft to the left lumbar spine and vertebral Between the paraspinal muscles, the nerve ends and implants into the left side of the recipient rat lumbar spinal dorsolateral.