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目的探讨载脂蛋白C3(ApoC3)基因启动子区-482C→T多态性与中国人DN的相关性。方法运用聚合酶链反应-限制性片段长度多态性技术(PCR-RFLP),在中国人群中对179例T2DM患者和60名正常对照者(NC)的ApoC3基因启动子区-482C→T的多态性进行检测,并比较各组间基因型频率和等位基因频率以及相关临床资料。结果 DN组(DN1+DN2)的T/T基因型频率及T等位基因频率高于DN0组(P<0.01),但DN2与ND1组间T/T基因型频率及T等位基因频率差异无统计学意义(P>0.05)。T2DM组中,T/T基因型组患者的血TG水平及24hUAlb均高于C/T和C/C基因型组者(P<0.01)。Logistic回归分析表明:ApoC3基因启动子区-482C→T多态性和DM病程与DN显著相关,-482T/T基因型是DN发生的危险因素;DN患者中,DM病程>5年是DN2发生的危险因素。结论在中国南方汉族人群中,ApoC3基因启动子区-482T/T基因型可能是DN发生的危险因素;病程>5年可能是DN进展的危险因素。
Objective To investigate the association between -482C → T polymorphism of apolipoprotein C3 (ApoC3) promoter region and DN in Chinese. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the polymorphism of -482C → T in 179 Chinese patients with T2DM and 60 normal controls (NC) Polymorphisms were detected, and genotype frequency and allele frequencies and clinical data were compared between groups. Results The frequency of T / T genotype and T allele in DN group (DN1 + DN2) were higher than that in DN0 group (P <0.01). However, the difference of T / T genotype frequency and T allele frequency between DN2 and ND1 group No statistical significance (P> 0.05). In T2DM group, TG level and 24hUAlb in T / T genotype group were higher than those in C / T and C / C genotype (P <0.01). Logistic regression analysis showed that: -482C → T polymorphism in ApoC3 promoter region and DM duration were significantly associated with DN, -482T / T genotype was a risk factor for DN; in DN patients, DM duration> 5 years was DN2 Risk factors. Conclusions -482T / T genotype of ApoC3 promoter region may be a risk factor for DN in Chinese Han population of southern China. Duration of> 5 years may be a risk factor for DN progression.