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鳙鱼卵透明带类物质加弗氏佐剂乳化后,对雌性家兔进行免疫注射。以被动血凝试验测定家兔抗鳙鱼卵透明带热溶性物质的血清效价。当雌兔血清抗体达一定水平时,与有生育力的雄兔进行交配,交配后于不同时间,将雌兔处死解剖,计算胎兔数及卵巢中黄体数,以精、卵结合抑制率为评价抗生育效应的指标。结果表明,家兔经鳙鱼卵透明带类物质免疫以后,血清抗体效价上升非常显著,于此同时,抗生育效应也显著增加。文章就鱼卵透明带类物质用于控制哺乳动物生育的可能性进行了讨论。
After being emulsified with zona pellucida plus Freund’s adjuvant, the female rabbits were immunized. Serum titer of zona pellucida thermosolytes in rabbit anti-mullet eggs was determined by passive hemagglutination test. When female rabbits serum antibody reached a certain level, mating with fertile male rabbits, mating at different times, the female rabbits were sacrificed and dissected to calculate the number of fetal rabbits and ovarian luteal number to fine, egg inhibition rate was Evaluation of anti-fertility effects indicators. The results showed that the antibody titer of rabbits increased significantly after the zona pellucida of rabbits immunized, meanwhile, the anti-fertility effect also increased significantly. The article discusses the potential of zona pellucida for controlling mammalian reproduction.