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目的探讨脑缺血预处理后3-硝基丙酸(3-NPA)对脑组织中caspase-3表达的影响。方法72只健康雄性SD大鼠,随机分成3组:脑缺血组24只,脑缺血预处理组24只,脑缺血预处理后3-NPA干预组24只。各组再按缺血时间分为6h、1、2和4天4个时间点。结果在缺血后相同时间点,脑缺血预处理组与脑缺血组比较神经功能缺损评分明显降低(P<0.05),梗死体积明显缩小(P<0.05),海马区caspase-3阳性细胞表达明显降低(P<0.05);脑缺血预处理后3-NPA干预组神经功能缺损评分、梗死体积、caspase-3阳性细胞表达较脑缺血预处理组明显降低,差异有显著性意义(P<0.05)。各组在脑缺血后6h出现caspase-3阳性细胞表达,1～2天达到高峰后逐渐下降。结论缺血预处理后3-NPA干预可进一步加强神经保护作用。
Objective To investigate the effect of 3-nitropropionic acid (3-NPA) on the expression of caspase-3 in brain after cerebral ischemic preconditioning. Methods Totally 72 healthy male SD rats were randomly divided into 3 groups: 24 in the ischemic group, 24 in the ischemic preconditioning group and 24 in the 3-NPA preconditioning group. The groups were divided into 6h, 1, 2 and 4 days according to the ischemic time at 4 time points. Results At the same time point after ischemia, the scores of neurological deficit in cerebral ischemic preconditioning group and cerebral ischemic group were significantly decreased (P <0.05), the infarct volume was significantly reduced (P <0.05), the expression of caspase-3 positive cells in hippocampus (P <0.05). Neurological deficit scores, infarct volume and expression of caspase-3 positive cells in 3-NPA group after cerebral ischemic preconditioning were significantly lower than those in cerebral ischemic preconditioning group, the difference was significant P <0.05). In each group, the expression of caspase-3 positive cells appeared at 6h after cerebral ischemia, and gradually decreased after reached peak at 1 ~ 2d. Conclusion The 3-NPA intervention after ischemic preconditioning can further enhance the neuroprotective effect.