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目的 观察P物质 (SP)对人脐静脉张力的调节及其对体外培养人脐静脉内皮细胞 (HUVEC)膜上Ca2 + 通道及胞浆内游离Ca2 + 浓度 [(Ca2 + )i]的调控作用。 方法 应用常规生理记录仪 ,记录SP对人脐静脉张力的影响 ,用共聚焦激光扫描显微术和膜片钳单通道记录技术对体外培养HUVEC胞浆内 (Ca2 + )i、膜上Ca2 + 通道开放情况进行观察。 结果 SP具有内皮依赖性舒张人脐静脉的作用 ,促使体外培养HUVEC胞膜上Ca2 + 通道开放和胞浆内 (Ca2 + )i明显升高。 结论 SP对人脐静脉张力的舒张作用可能通过HUVEC胞浆内储存的 (Ca2 + )i释放和膜上Ca2 + 通道开放 ,达到其调节效应
Objective To observe the regulation of substance P (SP) on the regulation of human umbilical vein tension and its role in the regulation of [Ca2 +] i in Ca2 + channels and cytoplasm of cultured human umbilical vein endothelial cells (HUVECs) in vitro . Methods The effects of SP on the tension of human umbilical vein were recorded by the conventional physiology recorder. The effects of SP on the intracellular Ca2 + i and the expression of Ca2 + in the HUVEC were observed by confocal laser scanning microscopy and patch-clamp recording. Channel open to observe the situation. Results SP had an endothelium-dependent relaxation effect on human umbilical vein, which promoted the opening of Ca2 + channels and the intracellular (Ca2 +) i in HUVEC cultured in vitro. Conclusion The diastolic effect of SP on human umbilical vein tension may be mediated by the release of (Ca2 +) i and the opening of Ca2 + channels in the cytoplasm of HUVEC