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目的建立养阴扶正胶囊中的橙皮苷、升麻素苷和5-O-甲基维斯阿米醇苷的HPLC测定方法。方法采用CAPCELL PAK C18反相色谱柱(150mm×4.6mm,5μm),以乙腈-0.2%磷酸为流动相进行梯度洗脱,柱温为30℃,流速为1.0ml/min,检测波长为256nm,进样量为5μl。结果橙皮苷在0.5~144.8μg·ml~(-1)范围内线性关系良好(r=0.9996),平均回收率为102.1%,RSD为1.2%;升麻素苷在1.2~148.7μg·ml~(-1)范围内线性关系良好(r=0.9994),平均回收率为101.5%,RSD为1.4%;5-O-甲基维斯阿米醇苷在2.3~138.3μg·ml~(-1)范围内线性关系良好(r=0.9995),平均回收率为100.5%,RSD为1.6%。结论本法操作简便、结果准确,可用于养阴扶正胶囊的质量控制。
Objective To establish an HPLC method for the determination of hesperidin, cimilipin and 5-O-methyl-Vaisalanthin in Yangyin Fuzheng Capsules. Methods A CAPCELL PAK C18 reversed-phase column (150 mm × 4.6 mm, 5 μm) was used with gradient elution with acetonitrile-0.2% phosphoric acid as the mobile phase. The column temperature was 30 ℃, the flow rate was 1.0 ml / min, the detection wavelength was 256 nm, Injection volume of 5μl. Results Hesperidin had a good linearity (r = 0.9996) in the range of 0.5 ~ 144.8μg · ml ~ (-1) with an average recovery of 102.1% and a RSD of 1.2%. Ciclofibir was stable in the range of 1.2 ~ 148.7μg · ml (-1), the average recovery was 101.5% and the RSD was 1.4%, while the linear range of 5-O-methyl-Vaisalanthin was 2.3 ~ 138.3μg · ml ~ (-1) 1) (r = 0.9995). The average recovery was 100.5% and the RSD was 1.6%. Conclusion This method is simple, accurate and can be used for the quality control of Yangyin Fuzheng Capsule.