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目的探讨紫外线照射对秀丽隐杆线虫形态、寿命和ROS含量的影响。方法线虫同步化培养至L4期,随机分为0 J/m2(对照组)、20 J/m2、40 J/m2、60 J/m2、80 J/m2、100 J/m2、120 J/m2和200 J/m2照射组共8组,用紫外交联仪进行照射。于照后1h、6 h、12 h和24 h,观察各组线虫形态变化;观察记录线虫死亡数和存活数,用于分析死亡率和寿命;于照后8 h、12 h和24 h,检测各组线虫体内ROS含量。结果与对照组比较,照后6～24 h,40 J/m2组线虫形态未观察到明显改变,而80 J/m2和120J/m2组中持续出现体型较小的线虫,且120 J/m2组明显多于80J/m2组。照后7 d各照射组线虫死亡率均高于对照组,且剂量越大死亡率越高,呈一定量效关系。各照射组线虫平均寿命均低于对照组,且照射剂量越大平均寿命越短。照后24 h内,80 J/m2和120 J/m2组线虫的荧光强度明显高于对照组;于照后8～24 h,120 J/m2组荧光强度均强于80 J/m2组。结论紫外线照射能明显诱导线虫形态学改变,影响线虫正常生长发育;大剂量照射可致线虫寿命缩短,推测线虫体内活性氧(ROS)含量增加可能是导致其寿命缩短的原因之一。
Objective To investigate the effect of UV irradiation on the morphology, life span and ROS content of Caenorhabditis elegans. Methods The nematodes were synchronized to L4 and were randomly divided into 0 J / m2 (control group), 20 J / m2, 40 J / m2, 60 J / m2, 80 J / And 200 J / m2 irradiation group, a total of 8 groups were irradiated with UV cross-linking instrument. The morphological changes of nematodes in each group were observed at 1h, 6h, 12h and 24 h after irradiation. The numbers of death and survival of nematode were observed and recorded for mortality and life expectancy. After 8 h, 12 h and 24 h, The contents of ROS in each group of nematodes were detected. Results Compared with the control group, the morphological changes of nematodes in the 40 J / m2 group were not observed after 6-24 hrs after irradiation, while the smaller ones were observed in the 80 J / m2 and 120 J / m2 groups with 120 J / m2 Group significantly more than 80J / m2 group. The mortality of nematodes in each irradiation group was higher than that in the control group on the 7th day after irradiation, and the higher the mortality was, the higher the dose was, the higher the dose-effect relationship was. The average life expectancy of nematodes in each irradiation group was lower than that of the control group, and the longer the irradiation dose, the shorter the average life expectancy. Fluorescence intensity of nematodes at 80 J / m2 and 120 J / m2 was significantly higher than that of control at 24 h after irradiation. Fluorescence intensity of 120 J / m2 at 8-24 h after irradiation was higher than that at 80 J / m2. Conclusion Ultraviolet irradiation can significantly induce the morphological changes of nematodes and affect the normal growth and development of nematodes. Large doses of irradiation can shorten the lifespan of nematodes. It is presumed that the increase of reactive oxygen species (ROS) in nematodes may be one of the reasons for its shortened life span.