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Liquid manure storage may contribute to methane(CH4)emission and this emission can be greatly reduced if appropriate management practices are applied. Biofiltration has been used in other fields for mitigating greenhouse gas(GHG)emission(e.g., landfill)and shown promise for mitigation CH4 emission from liquid manure storage. It has been reported that biofilter was capable of reducing 80% of CH4 emissions from manure storage. The CH4 removal efficiency is influenced by many factors, including CH4 and O_2 concentrations, temperature, moisture, composition of the filter bed, nutrient, and empty bed residency time(EBRT). Biological conversion of methane of a biofilter is a slow process due to the low water solubility of methane. The residence times(EBRT)between 5 min and 5 h have been used, whereas a typical EBRT of 25 s is used for common biofilter applications. Temperature at which methanotrophic bacteria are active ranges from 10℃ to 45℃. The maximum activity is found at around 30℃. The optimal filter bed water content depends on both the gas flow rate and the type of filter bed(soil, compost, etc.)and ranges from 30%-70% of the water holding capacity. Compost is the best material for filter bed. The optimal pH for methanotrophic bacteria is neutral to slightly acidic. Copper and nitrogen compounds especially nitrate are important nutrients to methanotrophic bacteria but their optimal concentrations have not been founded. Phosphorus and other elements such as potassium and manganese are reported to affect the performance of methanotrophic bacteria but need further confirmation.