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目的通过检测慢性乙型病毒性肝炎(CHB)、肝硬化以及肝细胞癌(HCC)患者肝组织内TLR4、TGF-β1、CCL20的表达的变化,探讨TLR4、TGF-β1、CCL20参与CHB的发生和发展的相关机制。方法选取临床标本104例,并将其分为4个组,分别为正常对照组26例、CHB组26例、肝硬化组26例以及HCC组26例。采用免疫组织化学方法检测TLR4、TGF-β1、CCL20在各组中的表达。结果免疫组化结果显示,TLR4、TGF-β1、CCL20在CHB、肝硬化以及HCC组肝组织中的表达均明显高于正常对照组(P<0.05)。TLR4和CCL20在HCC组的表达明显高于CHB组,差异有统计学意义(P<0.05),肝硬化与HCC组的表达差异无统计学意义(P>0.05)。TGF-β1在HCC组的表达明显高于肝硬化组和CHB组,差异均有统计学意义(P<0.05)。结论肝病患者的TLR4、TGF-β1、CCL20的表达水平与正常人相比均升高,在CHB的发生和发展过程中发挥着重要作用。
Objective To investigate the expression of TLR4, TGF-β1 and CCL20 in liver tissues of patients with chronic hepatitis B (CHB), cirrhosis and hepatocellular carcinoma (HCC), and to explore the role of TLR4, TGF-β1 and CCL20 in the pathogenesis of CHB And related mechanisms for development. Methods A total of 104 clinical specimens were selected and divided into 4 groups, which were 26 cases of normal control group, 26 cases of CHB group, 26 cases of liver cirrhosis group and 26 cases of HCC group. Immunohistochemistry was used to detect the expression of TLR4, TGF-β1 and CCL20 in each group. Results The results of immunohistochemistry showed that the expressions of TLR4, TGF-β1 and CCL20 in CHB, cirrhosis and HCC group were significantly higher than those in normal control group (P <0.05). The expression of TLR4 and CCL20 in HCC group was significantly higher than that in CHB group (P <0.05), but there was no significant difference between HCC and cirrhosis (P> 0.05). The expression of TGF-β1 in HCC group was significantly higher than that in cirrhosis group and CHB group (P <0.05). Conclusion The expression of TLR4, TGF-β1 and CCL20 in patients with liver disease are higher than those in normal people, and play an important role in the pathogenesis and development of CHB.