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AIM:To study the effects of Rheum tanguticumpolysaccharide_(-1)(RTP_(-1))on ulcerative colitis in rats inducedby 2,4,6-trinitrophene sulphonic acid(TNBS)and theirpossible mechanism.METHODS:RTP_1(200 mg·kg~(-1),ig)extracted from Rheumtanguticum Maxim.ex Regel was administrated to rats withcolitis induced by TNBS for 5 d,7 d,10 d and 14 d,respectively.The effects of RTP_1 and dexamethasone(DX,0.2 mg·kg~(-1),ig)were contrastively investigated.The MPOlevel and SOD activity were determined by chromatometry.The expansion and protein expression of CD4~+T lymphocytesisolated from colon mucosae and mesenteric lymph nodesof colitis rats were performed by immunohistochemicalanalysis and Western-blot methods.RESULTS:Treatments of RTP_1(200 mg·kg~(-1),ig)significantlyreduced diarrhea,mortality,colon mass,ulcer areas and MPOlevel in colon mucosae on days 5,7,10 and 14(5.2±1.4,5.4±0.7,5.2±1.8,P<0.05.3.4±0.8,P<0.01.16.1±12.1,P<0.01.31.8±8.6,17.7±5.3,12.7±4.1,P<0.05).The effectsof RTP_1 were similar to those noted above in DX group,butthere were no immunosupressive effects of DX in RTP_(-1)group,such as body mass loss,thymus and spleen atrophy.The decreased number and down-regulated protein levelsof CD4~+T cells isolated from the colon of colitis rats treatedwith RTP_1 were found.CONCLUSION:RTP_1 shows significantly protective effectsbut lower side effects on rats with colitis induced by TNBS.The mechanism may be due to the resistance to overexpansion of CD4.
AIM: To study the effects of Rheum tanguticumpolysaccharide _ (- 1) on ulcerative colitis in rats induced by 2,4,6-trinitrophene sulphonic acid (TNBS) and their sensible mechanism. METHODS: RTP_1 ~ (-1), ig) extracted from Rheumtanguticum Maxim. Regel was administrated to rats with colitis induced by TNBS for 5 d, 7 d, 10 d and 14 d, respectively. These effects of RTP_1 and dexamethasone (DX, 0.2 mg · kg ~ (-1), ig) were contrastively investigated.The MPOlevel and SOD activity were determined by chromatometry. The expansion and protein expression of CD4 ~ + T lymphocytesisolated from colon mucosae and mesenteric lymph nodes of colitis rats were performed by immunohistochemical analysis and Western- Significantly digested diarrhea, mortality, colon mass, ulcer areas and MPOlevel in colon mucosae on days 5, 7, 10 and 14 (5.2 ± 1.4, 5.4 ± 0.7, 5.2 ± 1.8, P <0.05.3.4 ± 0.8, P <0.01.16.1 ± 12.1, P <0.01.31.8 ± 8.6,17.7 ± 5.3,12.7 ± 4.1, P <0.05) .The effects of RTP_1 were similar to these noted above in DX group, butthere were no immunosupressive effects of DX in RTP _ (- 1) group, such as body mass loss, thymus and spleen atrophy. the decreased number and down-regulated protein levels of CD4 ~ + T cells isolated from the colon of colitis rats treated with RTP_1 were found. CONCLUSION: RTP_1 shows significantly protective effects but lower side effects on rats with colitis induced by TNBS. The mechanism may be due to the resistance to overexpansion of CD4.