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目的:探索缺氧诱导因子1α(HIF-1α)和诱导型一氧化氮合酶(iNOS)在胃癌组织中的表达水平及两者相关性。方法:采用免疫组化SP法检测85例胃癌组织和癌旁正常胃组织中的HIF-1α和iNOS的表达,并分析其相互关系。结果:HIF-1α和iNOS在胃癌组织中的表达水平(76.47%和71.76%)明显高于癌旁正常胃组织中(0)的表达水平(P=0.000),HIF-1α和iNOS的表达与胃癌的临床TNM分期、浸润程度和淋巴结转移有关(P<0.05),与组织学类型无关(P>0.05),HIF-1α的表达与iNOS的表达呈正相关r=0.761,P<0.05。结论:HIF-1α可能通过上调iNOS的蛋白表达促进肿瘤血管生成,HIF-1α与iNOS的高表达与胃癌的发生、浸润和转移密切相关。
Objective: To explore the expression of hypoxia-inducible factor-1α (HIF-1α) and inducible nitric oxide synthase (iNOS) in gastric carcinoma and their relationship. Methods: The expression of HIF-1α and iNOS in 85 cases of gastric cancer tissues and adjacent normal gastric tissues were detected by immunohistochemical SP method and the correlation was analyzed. Results: The expression levels of HIF-1α and iNOS in gastric cancer tissues (76.47% and 71.76%, respectively) were significantly higher than those in normal gastric tissues (P = 0.000) There was a significant correlation between the expression of HIF-1α and the expression of iNOS (r = 0.761, P <0.05), but not with the histological type (P> 0.05). Conclusion: HIF-1α may promote tumor angiogenesis by up-regulating the protein expression of iNOS. The high expressions of HIF-1α and iNOS are closely related to the occurrence, invasion and metastasis of gastric cancer.