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对金钱白花蛇及其伪、混品药材和原动物的Ｃｙｔｂ基因片段的序列分析发现，该基因片段在金钱白花蛇及其伪品间的差异远远大于金钱白花蛇种内个体间的差异，是理想的用于鉴别金钱白花蛇及其伪混品的分子遗传标记。在对Ｃｙｔｂ基因片段序列分析的基础上，设计了金钱白花蛇ＰＣＲ鉴别的一对高度特异性引物ＢｕＬ１和ＢｕＨ１。结果表明，该对引物在对金钱白花蛇的ＰＣＲ鉴别中，用６０℃～６５℃的复性温度，可以１００％检出金钱白花蛇，误检率和漏检率为０，并能在混合的药材粉末中检测出被检样品中是否含有金钱白花蛇组份。本研究还表明ＰＣＲ鉴别将有可能成为中成药复方组分鉴别的一种新手段。
The sequence analysis of the Cytb gene fragment of the golden snake and its pseudo, mixed herbs and protozoa found that the difference of this gene fragment in the snake and its counterfeit money was much greater than that in the snake, Is an ideal molecular genetic marker for the identification of Monascus and its fake hybrids. Based on the sequence analysis of Cytb gene fragment, a pair of highly specific primers BuL1 and BuH1 were identified by PCR. The results showed that the pair of primers could detect 100% white snake with a renaturation temperature from 60 ℃ to 65 ℃ in the PCR identification of S. auricula, the false detection rate and missed detection rate were 0, Of the medicinal powder in the sample to be tested whether it contains the money Baihuashe components. This study also shows that PCR identification will likely become a new means of identification of TCM compound components.