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目的:通过研究T细胞表达的共同刺激分子(CM)CD28、CD152、CD80、CD86在再生障碍性贫血(再障)小鼠的表达情况,探讨穿龙薯蓣皂苷治疗再障的作用机制。方法:用免疫介导法建立再障小鼠模型,随机分成模型组,高、中、低剂量组,雷公藤多苷组,环孢素组(Cs A组),并设正常组作为对照。造模第8天开始给药,正常组及模型组予等量0.9%氯化钠溶液。连续给药7d,眶丛静脉取血,对比外周血象,流式细胞术检测CD28、CD152、CD80、CD86的表达。结果:与正常组比较,模型组白细胞(WBC)、血红蛋白(HGB)及血小板(PLT)计数均显著减少(P<0.01),CD28、CD80、CD86的表达显著升高(P<0.05),CD152显著降低(P<0.05);与模型组比较,穿龙薯蓣皂苷各剂量组WBC、HGB及PLT计数均显著升高(P<0.05);中、高剂量组CD28、CD80、CD86的表达均显著减低,CD152的表达显著升高(P<0.05)。结论:穿龙薯蓣皂苷可能通过调节CM的表达从而发挥对再障的免疫调节作用。
OBJECTIVE: To investigate the effect of Diosmin on aplastic anemia in mice with aplastic anemia (AA) by studying the expression of costimulatory molecules (CDs) CD28, CD152, CD80 and CD86 on T cells. Methods: Aplastic anemia mice model was established by immune mediated method and randomly divided into model group, high, medium and low dose groups, tripterygium glycosides group and cyclosporine group (CsA group), and normal group as control group. On the 8th day after model administration, the rats in the normal group and model group were given the same amount of 0.9% sodium chloride solution. Continuous administration of 7d, orbital venous blood, compared with peripheral blood, flow cytometry CD28, CD152, CD80, CD86 expression. Results: Compared with the normal group, the counts of WBC, HGB and PLT in the model group were significantly decreased (P <0.01), the expressions of CD28, CD80 and CD86 were significantly increased (P <0.05) (P <0.05). Compared with the model group, the counts of WBC, HGB and PLT in each dose of Diosmin were significantly increased (P <0.05), and the expressions of CD28, CD80 and CD86 in medium and high dose groups were significantly increased CD152 expression was significantly increased (P <0.05). CONCLUSION: Diosgenin may play an immunomodulatory role in aplastic anemia by regulating the expression of CM.