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目的:通过向多介质土壤渗滤系统(MSL)的土壤层添加污泥,研究改进后的系统对工厂化甲鱼养殖废水的处理效果,分析系统内微生物群落的变化,以期部分揭示其可能的作用机理。创新点:工厂化甲鱼养殖废水排放量日益增大,且水体氨氮含量较高。目前市场上缺乏针对甲鱼养殖废水的处理技术,MSL系统对该废水的处理也未有报道。本文在MSL系统的基础上进行改良,并将其应用于甲鱼养殖废水处理上,提出一套有效的甲鱼养殖废水处理技术,并对MSL系统内微生物群落结构进行了分析。方法:向4套MSL小试装置中分别添加0%、5%、10%和20%污泥,研究其对工厂化甲鱼养殖废水的净化效果。试验中水质指标测定均按国家标准方法进行,系统内微生物群落结构采用聚合酶链反应-变性梯度凝胶电泳(PCR-DGGE)法测定。结论:MSL系统可有效地处理工厂化甲鱼养殖废水,向系统中添加20%污泥后处理效果更佳。添加20%污泥的系统内具有较高的硝化类细菌多样性和都较多的生物量。
OBJECTIVE: To investigate the effect of the improved system on the treatment of industrial turtle breeding wastewater by adding sludge to the soil layer of multi-media soil infiltration system (MSL), and to analyze the changes of microbial communities in the system in order to partially reveal its possible role mechanism. Innovative point: Factory turtle breeding wastewater emissions are increasing, and the higher ammonia nitrogen content. The current lack of treatment of turtle farming wastewater on the market, MSL system for the treatment of the wastewater has not been reported. Based on the MSL system, this paper is improved and applied to the treatment of turtle farming wastewater. An effective treatment technology of turtle farming wastewater is proposed and the microbial community structure in MSL system is analyzed. Methods: To 4 sets of MSL small pilot plant were added 0%, 5%, 10% and 20% of the sludge to study the purification of factory turtle farming wastewater purification effect. In the experiment, the water quality indicators were measured according to the national standard method. The microbial community structure was determined by PCR-DGGE. CONCLUSIONS: The MSL system can effectively treat industrial turtle farming wastewaters, adding 20% sludge to the system to provide better aftertreatment. The system of adding 20% sludge has higher nitrifying bacteria diversity and more biomass.