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目的比较吉西他滨和顺铂对人胆管癌细胞放射增敏怍用的差异。方法取指数生长期的人胆管癌细胞系(QBC939),采用细胞克隆形成分析法分别检测吉西他滨和顺铂的单药毒性,确定半数抑制药物浓度(IC50)作为下一步实验的药物浓度。将QBC939细胞分为对照组、单纯药物组、单纯照射组及联合治疗组。单纯药物组、联合治疗组分别分为吉西他滨亚组和顺铂亚组。X射线照射剂量为0Gy、1Gy、2Gy、4Gy、6Gy、8Gy、10Gy,采用多靶单击数学模型拟合细胞存活曲线,放射增敏比(SER)为达到相同之处生物效应时,单纯照射的剂量与联合治疗组的剂量之比。结果吉西他滨对QBC939细胞的IC50为11.0nM/L.顺铂对QBC939细胞的IC50为0.4μM/L。以D为标准计算放射增敏比,吉西他滨的SER_(?)分别2.64,顺铂的SER_(?)分别1.01。结论吉西他滨较顺铂对QBC939细胞具有更强的增敏作用。
Objective To compare the difference of radiosensitization of human cholangiocarcinoma cells between gemcitabine and cisplatin. Methods The human cholangiocarcinoma cell line QBC939 was taken exponentially. The single-agent toxicity of gemcitabine and cisplatin was detected by cell clone formation assay. The half-inhibitory concentration (IC50) was determined as the drug concentration for the next experiment. QBC939 cells were divided into control group, simple drug group, simple irradiation group and combined treatment group. Simple drug group, combination therapy group were divided into gemcitabine subgroup and cisplatin subgroup. X-ray irradiation dose was 0Gy, 1Gy, 2Gy, 4Gy, 6Gy, 8Gy, 10Gy, using a multi-target single-click mathematical model fitting cell survival curve, radiosensitization ratio (SER) to achieve the same biological effects, Of the dose and the combined treatment group dose ratio. Results The IC50 of gemcitabine on QBC939 cells was 11.0 nM / L and the IC50 of cisplatin on QBC939 cells was 0.4 μM / L. The radiosensitivity was calculated by D, the SER_ (?) Of gemcitabine was 2.64, and the SER_ (?) Of cisplatin was 1.01 respectively. Conclusion Gemcitabine has stronger sensitizing effects on QBC939 cells than cisplatin.