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Miniature inverted-repeat transposable elements(MITEs) are a type of DNA transposon frequently inserted into promoters, untranslated regions(UTR), introns, or coding sequences of genes. We found a 276-bp tourist-like MITE insertion in the 3′-UTR of a 16.9 k Da small heat shock protein gene(TaH SP16.9-3A) on chromosome 3A of common wheat. Haplotype analysis revealed two haplotypes, s HSP-W(wild type without MITE insertion) and s HSP-M(mutant with MITE insertion), present in wheat germplasm. Both semiquantitative PCR and quantitative real-time PCR analyses showed increased transcription levels of TaH SP16.9-3A in s HSP-M compared with those of s HSP-W after heat treatment at 42 °C. It appeared that the MITE insertion into the 3′-UTR enhances the transcription of TaH SP16.9-3A.
We found a 276-bp tourist-like MITE insertion in the 3’- UTR of a 16.9 kDa small heat shock protein gene (TaH SP16.9-3A) on chromosome 3A of common wheat. Haplotype analysis revealed two haplotypes, s HSP-W (wild type without MITE insertion) and s HSP-M with MITE insertion), both in semiquantitative PCR and quantitative real-time PCR analyzes showed increased transcription levels of TaH SP16.9-3A in s HSP-M compared with those of HSP-W after heat treatment at 42 ° C. It has that the MITE insertion into the 3’-UTR enhances the transcription of TaH SP16.9-3A.