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本实验室经过反复探索对传统的精子畸形试验法进行了改进,减化了试验步骤。 改进方法:小白鼠染毒第35天,用颈椎脱臼法处死小鼠,取出一侧附睾(附睾尾部尽量长些),用生理盐水冲洗两遍,以除去表面的杂质。然后沿附睾纵轴剪开,放入装有0.25ml 2％伊红染液的小试管内,用毛吸管反复吸打4～5次,取出附睾后继续用毛吸管吸打5～6次,染色5min后制片,自然干燥后,甲醇溶
After repeated exploration in our laboratory, the traditional test method of sperm deformity was improved, which reduced the test procedure. Improvement method: mice on the 35th day of exposure, the mice were sacrificed by cervical dislocation, remove the side of the epididymis (epididymis tail as long as possible), rinse twice with saline to remove surface impurities. Then cut along the longitudinal axis of the epididymis, placed in a small test tube containing 0.25ml 2% Eosin dye, repeatedly suck the wool tube 4 to 5 times, remove the epididymis and continue to use wool pipette 5 to 6 times, After dyeing 5min production, natural drying, dissolved in methanol