Characterization and Comparison of Ochratoxin A-Ovalbumin （OTA-OVA） Conjugation by Three Methods

• 作 者: WANG Xi-chun BAO Ming WU Jin-j
• 机 构: CollegeofAnimalScienceandTechnology,AquaticTechnologyPromotionStationofAnhuiProvince,CollegeofVeteri
• 出 处: 农业科学学报：英文版
• 发表日期: 2014年5期
• 关键词: 赭曲霉毒素A MALDI-TOF-MS 卵清蛋白 基质辅助激光解吸电离质谱 共轭 抗原分子 表征 半抗原 ochratoxin A conjugated an
• 基金项目: Acknowledgements This work was supported by the National High-Technology Research and Development Program of China （2007AA10Z429） and the Stabling and Introducing Talents Fund Program of Anhui Agricul

In order to generate an antibody against a small hapten molecule, the hapten is cross-linked with carrier protein to make it immunogenic. In this study, the hapten(ochratoxin A, OTA) was coupled to ovalbumin(OVA) by an active ester reaction. To develop a