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目的研究不同剂量的PPDA诱导免疫耐受的反应曲线和免疫耐受过程中Th1细胞和Th2细胞的调节关系。方法用不同剂量的PPDA建立3个不同的动物实验模型,连续12周,用临床皮肤评分法得到这3个实验模型免疫反应的曲线,并比较其强弱;在实验的4个阶段取其活组织用ELISA法进行IL-10,IL-12,IL-1α细胞因子测定,分析IL-10和IL-12在免疫耐受发生中的调节关系。结果3组别各周平均反应分值无差别,在第7和9周实验剂量交叉时,大剂量组的皮肤反应大于小剂量组。IL-10皮肤含量在实验第6周达高峰,a1和a2组的IL-10含量在第8周较第6周明显下降,差异有统计学意义(P<0.05);a1组的IL-10值在第12周较第6周明显下降,差异有统计学意义(P<0.05);a1组在第12周的IL-10含量低于a2组。第6周的IL-12含量大于0时刻,差异有统计学意义(P<0.05);第8周IL-12含量较第6周明显上升;a1组在第12周的IL-12大于a2组。IL-1α各组各周之间差异均无统计学意义(P>0.05)。结论在本试验条件下,小剂量抗原较大剂量抗原更易使Th1向Th2倾斜;IL-10可能调节Th2型免疫反应。
Objective To study the response curves of different doses of PPDA induced immune tolerance and the regulation of Th1 and Th2 cells during immune tolerance. Methods Three different experimental animal models were established with different doses of PPDA for 12 consecutive weeks. The curves of immune responses of the three experimental models were obtained by clinical skin score method. The four experimental stages were selected for their immune response. IL-10, IL-12 and IL-1α cytokines were measured by ELISA, and the regulation of IL-10 and IL-12 in immune tolerance was analyzed. Results There was no difference in mean weekly response scores between the three groups. At the 7th and 9th week of experimental dose crossover, the skin response of the high dose group was greater than that of the low dose group. The content of IL-10 in the skin reached its peak at the 6th week of the experiment, and the content of IL-10 in the a1 and a2 groups decreased significantly at the 8th week compared with the 6th week (P <0.05) The values in the 12th week were significantly lower than those in the 6th week (P <0.05). The content of IL-10 in the a1 group was lower than that of the a2 group in the 12th week. The level of IL-12 in the 6th week was greater than that in the 0th, the difference was statistically significant (P <0.05); IL-12 content in the 8th week was significantly higher than that in the 6th week . There was no significant difference between each week of IL-1α (P> 0.05). Conclusions Under the conditions of this experiment, it is easier for Th1 to Th2 to be tilted by the low dose of antigen than the high dose of antigen. IL-10 may regulate the Th2-type immune response.