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以凝集活性为指标,纯化MW6000的糖蛋白(6KDP),为了质量评价,以EIA法对6KDP进行定量,并观察了6KDP的镇吐活性。 ①凝集素的制备:以80％饱和硫酸铵使3％NaCl可溶部分沉淀后,以精制水透析,得球蛋白组分。以家兔红细胞凝集为指
The MW6000 glycoprotein (6KDP) was purified using the agglutination activity as an index. For quality assessment, 6KDP was quantified by EIA and the antiemetic activity of 6KDP was observed. Preparation of a lectin: The 3% NaCl soluble fraction was precipitated with 80% saturated ammonium sulfate and dialyzed against purified water to obtain a globulin fraction. With rabbit red blood cell agglutination means