论文部分内容阅读
目的 观察培养的施万细胞 (SCS)在平面或聚乙醇酸 (PGA)纤维的立体网架上迁移特性。 方法 用光、电镜技术观察在不同培养环境和培养时间中 ,SCS在平面或立体网架上的形态变化。 结果 平面上 SCS一般为长梭形 ,同群细胞间以突起相连接 ;同群细胞迁移有方向和速率一致的特征。立体网架上细胞有球形和长橄榄形两种 ,细胞间也互相接触 ;细胞迁移速度快于平面 ,纤维上的长橄榄形细胞快于球形细胞 ;长橄榄形细胞长轴往往与纤维呈一定夹角 ,有包裹纤维的趋势。 结论 PGA在 SCS生长中具有良好的生物相容性 ;与平面相比 SCS更易在立体网架的纤维上迁移 ,长橄榄形的 SCS有包裹纤维的趋势。
Objective To observe the migration characteristics of cultured Schwann cells (SCS) on the three-dimensional network of planar or polyglycolic acid (PGA) fibers. Methods The morphological changes of SCS on planar or three-dimensional grids were observed under light and electron microscopes in different culture environments and culture time. Results The SCS was generally long fusiform in plane and connected with protuberances in the same group of cells. The same direction and velocity of the same group of cells were migrated. The cells on the three-dimensional network are spherical and long olivary, and the cells are also in contact with each other. The cells migrate faster than the plane, and the long olivary cells on the fiber are faster than the spherical cells. The long olivoid cells often have a long axis with the fibers Angle, wrapped fiber trend. Conclusions PGA has good biocompatibility in the growth of SCS. Compared with plane SCS, PGA is more likely to migrate on the fibers of the three-dimensional network. The long olive-shaped SCS has the tendency of wrapping fibers.