论文部分内容阅读
目的 观察比较阿霉素 ( ADM)化疗与 43℃加热化疗对耐药人肝癌细胞模型 - 772 1 /Adm(以下简称 772 1 /Adm细胞 )的敏感性及细胞内药物浓度的影响。方法 以人肝癌细胞模型 -772 1 /Adm为研究对象 ,采用水浴加温法、体外细胞毒试验 ( MTT法 )、流式细胞技术 ,观察阿霉素( ADM)化疗与加热化疗后细胞的存活率及细胞内阿霉素浓度的变化。结果 ( 1 )阿霉素化疗、加热化疗 30、60 min后 772 1 /Adm细胞存活率分别为 70 .2 %、40 .8%和 60 .2 %、37.4% ;( 2 )流式细胞仪检测显示阿霉素化疗、加热化疗 30 min后细胞内阿霉素浓度分别为 41 .3%、92 .0 %。结论 加热可以显著对抗 772 1 /Adm的耐药性 ,提高其对阿霉素的敏感性 ,这与加热提高了细胞内药物浓度有关。
Objective To observe the effect of adriamycin (ADM) chemotherapy and 43 °C heating chemotherapy on the sensitivity of human hepatoma cell model-7721/Adm (hereinafter referred to as 772 1 /Adm cells) and the influence of intracellular drug concentration. Methods The human hepatoma cell model -772 1 /Adm was used as the research object. The temperature of cells after chemotherapy and thermal chemotherapy with adriamycin (ADM) was observed by water bath heating, in vitro cytotoxicity assay (MTT) and flow cytometry. Rates and changes in intracellular adriamycin concentrations. Results (1) The survival rate of 772 1 /Adm cells after adriamycin chemotherapy and heating chemotherapy 30 minutes and 60 minutes were 70. 2%, 40.8%, 60. 2% and 37.4% respectively; (2) Flow cytometer The detection showed that the intracellular adriamycin concentration after adriamycin chemotherapy and heating chemotherapy was 41. 3% and 92.0%, respectively. Conclusion Heating can significantly counteract the drug resistance of 7721/Adm and increase its sensitivity to doxorubicin, which is related to the increase of intracellular drug concentration by heating.