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目的:探讨乙肝病毒DNA(HBV DNA)和前S1抗原(Pre-S1 Ag)在产前检查中的意义。方法:从产前检查孕产妇标本中筛选出乙肝表面抗原阳性[HBsAg(+)]标本417例,乙肝病毒血清标志物(HBV-M)全阴性标本50例为对照,分别采用时间分辨荧光免疫分析法(TRFIA)检测HBV-M,荧光定量聚合酶链反应(FQ-PCR)检测HBV DNA和酶联免疫吸附法(ELISA)检测Pre-S1 Ag,对结果进行对比分析。结果:417例HBsAg(+)孕产妇中,HBV DNA与Pre-S1 Ag总阳性率比较,差异无统计学意义(P>0.05);HBV DNA与Pre-S1 Ag阳性率均高于HBeAg(-)组(P<0.05)。在185例HBV DNA(+)患者中有61例Pre-S1 Ag为阴性,232例HBV DNA(-)患者中有45例Pre-S1 Ag为阳性。50例血清标志物全阴性标本的HBV DNA与Pre-S1 Ag均为阴性。结论:在产前检查中增加HBV DNA与Pre-S1 Ag检测是对乙肝血清标志物的良好补充,联合检测有助于准确判断孕产妇体内是否存在乙肝病毒复制,以便临床及时采取阻断措施,减少病毒母婴传播。
Objective: To investigate the significance of HBV DNA and Pre-S1 Ag in prenatal examination. Methods: 417 cases of HBsAg positive [HBsAg (+)] specimens were screened from prenatal maternal specimens and 50 cases of HBV-M negative specimens were used as controls, respectively. Time-resolved fluorescence immunostaining HBV DNA was detected by TRFIA, HBV DNA was detected by FQ-PCR and Pre-S1 Ag was detected by enzyme linked immunosorbent assay (ELISA), and the results were compared. Results: The positive rates of HBV DNA and Pre-S1 Ag in 417 HBsAg (+) pregnant women were not significantly different (P> 0.05). The positive rates of HBV DNA and Pre-S1 Ag were higher than those of HBeAg (- ) Group (P <0.05). Pre-S1 Ag was negative in 61 of 185 HBV DNA (+) patients and 45 of Pre-S1 Ag positive in 232 HBV DNA (-) patients. All 50 serum samples were negative for HBV DNA and Pre-S1 Ag. Conclusion: Increasing HBV DNA and Pre-S1 Ag in prenatal examination is a good complement to serum markers of hepatitis B. Combined testing can help to accurately determine the presence of hepatitis B virus replication in pregnant women in order to promptly take blocking measures in clinical practice, Reduce the mother to child transmission of the virus.