TRANSFORMING ACTIVITY OF DNA FROM HUMAN ESOPHAGEAL CANCER AND THE IDENTIFICATION OF THE TRANSFORMING

• 作 者: 梁克理 李则孝林焯唐 高其鑫 蒋东霞 李锦洲 刘东亮 陈渊卿 顾健人
• 机 构: Henan Tumor Institute, Zhengzhou,Henan Tumor Institute, Zhengzhou,Henan Tumor Institute, Zhengzhou,H
• 出 处: Chinese Journal of Cancer Research
• 发表日期: 1990年04期
• 关键词: DNA esophageal nude fibrous suffered sarcoma Plasmid squamous injected transfect

Rat-1 cells were transfected with DNA from human esophageal cancer 2K, 4K, 6K, 7K. 8K. The transforming foci were obtained and the transforming cell lines were established. The cell lines can form larger colony in soft agar. Those nude mice injected subcutaneously with the cells suffered from larger fibrous sarcoma. This indicates that the cell lines have carcinogenicity. The experimental results suggest that human DNA sequence and human Ha-ras special 616Kb (BamHI) band are present in the DNA of the transforming cells. The over-expression of ras gene products P21 were found in the tissues of exophageal cancer, the tissues adjacent to tumor and the transforming cells.

Rat-1 cells were transfected with DNA from human esophageal cancer 2K, 4K, 6K, 7K. 8K. The transforming foci were obtained and the transforming cell lines were established. The cell lines can form larger colony in soft agar. The experimental results suggest that human DNA sequence and human Ha-ras special 616Kb (BamHI) band are present in the DNA of the transforming cells. sub- overlying the cells suffer from larger fibrous sarcoma. This indicator that the cell lines have carcinogenicity. -expression of ras gene products P21 were found in the tissues of exophageal cancer, the issues adjacent to tumor and the transforming cells.