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以国内外的10个双孢菇菌株为试材,利用ISSR分子标记技术对其进行亲缘关系分析。结果表明:从117个ISSR引物中筛选出11个条带清晰重复性好的引物,对10个双孢菇菌株的基因组DNA进行扩增,共获得73条清晰条带,其中多态性条带51条,多态性位点百分率为69.86%。利用NTSYSpc 2.10软件计算菌株间Nei遗传距离为0.5479~0.9589,以平均连锁法(UPGMA)进行聚类分析,可将10个双孢菇菌株分为4个类群:类群Ⅰ为国内的2个菌株258和2796;类群Ⅱ是引自荷兰的HRLM;类群Ⅲ包括引自美国的L901、5113、XXX和2200;类群Ⅳ包括引自荷兰的H901、F3F和HA15。引自美国和荷兰的双孢菇菌株之间亲缘关系较近,相似性达0.85以上,而与我国的双孢菇亲缘相似性仅为0.61。因此,国外优质双孢菇菌株的引进对于增加我国双孢菇遗传多样性具有重要作用。
Ten strains of Agaricus bisporus at home and abroad were used as materials to analyze the genetic relationship using ISSR molecular marker technology. The results showed that 11 clear and repeatable primers were screened out from 117 ISSR primers, and the genomic DNA of 10 Agaricus bisporus strains was amplified. A total of 73 clear bands were obtained, of which 51 polymorphic bands , The percentage of polymorphic loci was 69.86%. The Nei genetic distance between isolates was 0.5479 ~ 0.9589 using NTSYSpc 2.10 software. UPGMA cluster analysis showed that 10 Agaricus bisporus strains were divided into four groups: Group Ⅰ was domestic 258 strains and 2,796 strains ; Group II is derived from the Netherlands HRLM; Group III includes L901, 5113, XXX and 2200 from the United States; Group IV includes H901, F3F and HA15 from the Netherlands. The relatives between the strains of Agaricus bisporus in the United States and the Netherlands are close, the similarity is more than 0.85, while the relative similarity with Agaricus bisporus in China is only 0.61. Therefore, the introduction of high quality Agaricus bisporus abroad plays an important role in increasing the genetic diversity of Agaricus bisporus in China.