论文部分内容阅读
目的:建立肠炎清中小檗碱的含量测定方法。方法:HPLC色谱柱为Diamonsil C18;以乙腈-0.01 mol/L庚烷磺酸钠与0.02 mol/L磷酸二氢钾等量混合溶液(用10%磷酸调节pH值3.0)(32∶68)为流动相,检测波长为265 nm。结果:小檗碱在0.0744～0.744μg范围内线性关系良好,相关系数r=0.9999,平均回收率为99.1%(RSD=1.75%)。结论:本法快速、准确,重复性好,可作为肠炎清的质控指标之一。
Objective: To establish a method for determination of berberine in enteritis. METHODS: The HPLC column was Diamonsil C18. The mixture of acetonitrile-0.01 mol / L heptane sulfonate and 0.02 mol / L potassium dihydrogen phosphate (adjusted to pH 3.0 with 10% phosphoric acid) (32:68) was Mobile phase, detection wavelength of 265 nm. Results: Berberine showed good linearity in the range of 0.0744 ~ 0.744 μg with a correlation coefficient of 0.9999 and an average recovery of 99.1% (RSD = 1.75%). Conclusion: This method is rapid, accurate and reproducible. It can be used as one of the quality control indicators of enteritis.