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选用抗旱性有差异的6份甘薯栽培品种为试验材料,在田间鉴定抗旱性的基础上,通过室内PEG-6000模拟水分胁迫,测定胁迫下与抗旱性相关的9个生理生化指标,采用抗旱指数和隶属函数相结合的方法筛选与抗旱性密切相关的生理生化指标,对其抗旱性进行评价。结果表明:经田间抗旱鉴定,广薯87和徐薯22的抗旱性强,日紫0602和宁紫薯1号的抗旱性差;在PEG-6000模拟水分胁迫下,甘薯叶片NOS、CAT、T-AOC和T-SOD活性随着PEG浓度增高,其活性增强;Pro、MDA、Prot和T-AA含量也增加;尤其在25%PEG-6000胁迫处理24 h,甘薯叶片NOS、T-AOC、Pro的相对值与抗旱指数呈显著正相关,RWC相对值与抗旱指数呈极显著正相关,这4个指标的加权隶属函数值D与抗旱指数呈显著正相关(r=0.8944);因此,可用这4个指标的D值对甘薯抗旱性进行初步鉴定。
Six sweetpotato cultivars with different drought resistance were selected as experimental materials. Nine drought-resistant physiological and biochemical indexes under drought stress were determined by in-situ PEG-6000 simulated water stress. Based on drought resistance index And the subordinate function to screen the physiological and biochemical indexes which are closely related to the drought resistance and to evaluate its drought resistance. The results showed that the drought resistance of Guangshu 87 and Xushu 22 was strong, and the drought resistance of Zi Zi 0602 and Ningxiaxu 1 was worse than that of Ningzhu 1 by field drought resistance test. Under PEG-6000 simulated water stress, NOS, CAT, T- The activities of AOC and T-SOD increased with the increase of PEG concentration. The contents of Pro, MDA, Prot and T-AA also increased. Especially under 24% PEG-6000 treatment, the NOS, T-AOC, The relative value of drought resistance index was positively correlated with the drought resistance index, and the RWC relative value had a significant positive correlation with the drought resistance index. The weighted membership function value D of these four indicators had a significant positive correlation with the drought resistance index (r = 0.8944); therefore, D value of four indicators of sweet potato drought resistance preliminary identification.